Vol. 7, Issue 2, Part I (2025)

The present investigation describe the anti-allergic effect of a poly-herbal extract which was established by three distinct methods using KU812 cell lines and IL33 release inhibition by HaCaT cells. Basophil inactivation leading to suppression of histamine release, inhibition of PGD2 and the release inhibition of IL33 by HaCaT cells. All the three studies clearly show the effect of the poly-herbal exact in concentration dependent manner. The finding thus obtained from two separate cells lines that release different mediators responsible for allergic manifestation confirm the strong pharmacological effect of the poly-herbal extract and its possible medical value in the treatment of allergic disorders. Further, the no cytotoxic effect of the poly-herbal extract to both the cell lines by MMT was also established. The findings of the investigation is described in the article.