Vol. 6, Issue 2, Part B (2024)

Citrus medica and Zingiber officinale has a variety of bioactive components that have several therapeutic uses. This study evaluated the bioactive properties of aqueous and ethanolic extracts derived from the fruit peel of Citrus medica and the rhizome of Zingiber officinale, assessing their antioxidant, antibacterial, and anthelmintic effects. Dried, ground rhizomes of Z. officinale and fresh fruits peel of C. medica were extracted by maceration and Soxhlet extraction method with ethanol and water, respectively. DPPH assay and total antioxidant status (TAS) by a colorimetric method was performed to analyses the antioxidant properties. Total phenolic content from ethanol extracts were found the highest 13.5 ± 2.26 and 66±0.14 μg GAE/g dry weight, while total flavonoid content ethanol extracts were found 183.17±8.12 and 464±0.30 of the Z. officinale and C. medica respectively. Disc diffusion method, with minor modifications was used for the evaluation of in-vitro antimicrobial activity against S. aureus, and E. coli. S. aureus was affectively controlled by the Z. officinale and C. medica aqueous and ethanol extract at 10 µl/ml and 5 µl/ml. The mean diameters of inhibition zone of Z. officinale and C. medica extracts for 10 µl/ml and 5 µl/ml were 14.0±1.0, 12.67±0.58, and 15.2±0.66, 13.2±1.8 mm, respectively. Anthelmintic study was conducted using Eisenia fetida as a test organism, revealing a significant anthelmintic effect in the C. medica and Z. officinale extract compared to the standard drug. Paralysis of earth-worms occurred within 26 and 16 min when exposed to concentrations of 30 mg/ml while 66 and 46 min for 10 mg/ml of Z. officinale and C. medica extracts respectively. This study suggests that Z. officinale rhizomes and C. medica fruit peel extract may use as anthelmintic, antibacterial, and antioxidant agents. As a result, they highlight the natural source's potential uses in a variety of applications.