Vol. 6, Issue 2, Part B (2024)

Through LC, the segments of a specimen are isolated by how they seep into the fixed and mobile phases. The info gotten by mass spectroscopy discovers and teaches about the structure of each part. The method called Comprehensive 2-Dimensional liquid chromatography (LC×LC) is new. The first instance that LC x LC was remarkable to the effect that it pointed out the sample's separations using two different columns, unlike the classical multidimensional chromatography. When met with the mixtures that are too hard to be resolved by other means, LC technicians like to this technique. This instrument has gained power when a mass of a tertiary dimension that a LC x LC system involves is added to a mass spectrometer. This article puts the 2D LC-MS equipment under the microscope, as well as the differences between LC and 2d LC and their applications are the objectives of this study.