Nsonde Ntandou FG, Sekhoacha M, Nkoua Badzi C, Molefe D, Etou Ossibi AW, Elion Itou RDG, Motlalepula Matsabisa G, Abena AA
For more effective in the treatment of malaria by plants in traditional medicine, a scientific validation on the efficacy and tolerability of the treatments is essential. This study aims firstly to evaluate the knowledge of health tradipractitioners on the pathophysiological manifestations that helped to establish a clinical diagnosis of malaria; secondly to identify the different recipes of the plants used against malaria with a view to select plants to be evaluated for their citotoxicity and antiplasmodial effect. An ethnobotanical and clinical knowledge of malaria surveys have been conducted in Nkayi and Gamboma semi-rural cities. Five plants were selected and submitted to aqueous maceration and sequential maceration using polar croissant solvent system: hexane, dichlorometane (DCM), mix v dichloromethane / v methanol (MeOH) and methanol. Antiplasmodial assay was conducted with D10 sensitive Plasmodium falciparum strain. Cytotoxicity was assessed at Vero cells line. Cassia siamea bark aqueous, hexane and DCM / MeOH extracts, Quassia africana roots aqueous and MeOH extracts, Nauclea latifolia leaves DCM/MeOH, MeOH and roots DCM/MeOH extracts, Rauvolfia vomitoria leaves DCM/MeOH, MeOH extracts are highly active(IC50 < 6,25 μg / ml). Some of these active extracts are completely devoided of the cytotoxicity, others are weakly cytotoxic (44, 09 ± 1, 47 μg / ml ≤ CC50 ≤ 120, 5 ± 1, 94 μg / ml; 14.22 > SI > 19, 28). Many chemical molecules of these plants are responsible for activity.
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